Mass Spectrometry

Overview of Services

This Core is housed within the MUSC Mass Spectrometry Facility which provides expertise, services, education, and training to enhance biomedical research endeavors through mass spectrometry-based proteomics. Currently there are over 50 investigators which utilize the facility for protein identification and characterization. Protein analysis includes in-gel or in-solution protease digestion, chromatographic separation and tandem mass spectrometric analysis of the resulting peptides, and interpretation of MS/MS data using Sequest , Mascot,  Protein Pilot, MaxQuant, and other search algorithms. The facility also assists in the development of customized applications for the isolation, detection and characterization of posttranslationally modified peptides (e.g. phosphorylation, glycosylation, oxidation, glutathionylation, and O-GlcNAc modification). With the recent acquisition of the Orbitrap Elite Mass Spectrometer we are expanding our services to couple quantitative approaches (SILAC, iTRAQ®, ICAT®, TMT®) to modification-specific experiments (eg.,phosphoproteomics, redox proteomics). We are developing methodology to analyze alterations in posttranslational regulation that impact signal transduction, epigenetic modulation, and the response to therapeutics with the goal of enabling investigators to discover molecular mechanisms underlying disease progression and therapeutic responses that may not be revealed through genomic studies.


Services: MALDI-TOF MS, LC-MS, and LC-MS/MS tandem mass spectrometry analyses are offered for protein analysis.  Protein identification services include in-gel or in-solution protease digestion, chromatographic separation and tandem mass spectrometric analysis of the resulting peptides, and interpretation of MS/MS data using Sequest® or Mascot® software. The facility will also assist in the development of customized applications for the isolation, detection and characterization of posttranslationally modified peptides (e.g. phosphorylation, glycosylation, oxidation, glutathionylation, and O-GlcNAc modification).  Sites of modification are verified by manual inspection of the data.  Please consult facility staff for feasibility and pricing of quantitative proteomic experiments (iTRAQ or SILAC), the implementation of specialized approaches with quantitative proteomics (e.g. phosphoproteomics, O-GlcNAc proteomics), and MALDI-imaging mass spectrometry for tissue imaging experiments.


       


Leadership

Director:  Lauren E. Ball, Ph.D.; 843-792-4513, ballle@musc.edu


Lab Manager:  Jennifer Bethard, M.S.; 843-792-8637, bethard@musc.edu


Staff: Susana Comte-Walters and Scott Hoover


Location and hours of operation

Hours Location

Available for Consults         

Wednesday 1:00PM

CRI 314

305 Children’s Research Institute

Charleston, SC 29425

Links and Resources

  1. Mass Spectrometry Facility 

Contacts

Name Role Phone Email Location
Jennifer Bethard
Lab Manager
 
843-792-8637
 
bethard@musc.edu
 

 

 

 

 

 

Services

Name Description Price
Analysis Request 

To submit this request:

  1. Locate service in services menu at bottom of page
  2. Click Initiate Service
  3. Complete the requested details of your project
  4. Submit to Core (no UDAK required to submit)
  5. Core will be in contact to set-up a meeting or to begin your project.
 
*
Gel Electrophoresis Facility Request 

To submit a request to the Gel Proteomics Core click on initiate request and follow the steps to fill out the request form. You will be contacted by the core.

 
*
Training Request 

Initiate a training request to recieve training on any of these three pieces of equipment.

  • Bruker Autoflex III Imaging MALDI-TOF-TOF
  • Bruker Autoflex III MALDI-TOF
  • Thermo LTQ  (ESI-linear IT)
 
*
LC-MS/MS medium gradient 

moderately complex mixture - medium gradient

Orbitrap Elite

Digestion Included

 
*
1D Gel + Electroblot 10-15cm    *
1D Gel + Electroblot 17-26cm    *
1D Gel 10-15cm    *
1D Gel 17-26cm    *
2D Gel + Electroblot 10-15cm    *
2D Gel + Electroblot 17-26cm    *
2D Gel 10-15cm    *
2D Gel 17-26cm    *
2D LC-MS/MS (10 fractions) 

(10 fractions) ($125/salt step/sample)

 
*
2D LC-MS/MS (5 fractions) 

(5 fractions) ($125/salt step/sample)

Digestion included

 
*
4-Plex iTraq 

Contact Lauren Ball for consulation and pricing

 
*
8-plex iTRQ    *
Blue Native Page 10cm    *
Blue Native Page 10cm with Electrotransfer    *
Colloidal Coomasie Brilliant Blue 10-15cm    *
Colloidal Coomasie Brilliant Blue 17-26CM    *
De Novo Sequencing analysis    *
Deep Purple Gel Stain 10-15cm    *
Deep Purple Stain 17-26cm    *
Determination of occupancy 

To determine % occupancy of a modfication

 
*
DIGE Minimal CyDyes/50ug of protein    *
DIGE Saturation CyDyes/50ug Protein    *
Iodo TMT    *
LC-MS/MS fast gradient 

(gel spot/band) fast gradient

Digestion included

 
*
LC-MS/MS long gradient 

complex mixture or PTM analysis - long gradient

Digestion included

 
*
LTQ Identification 

gel band or spot protein identifications

low resolution data

 

 
*
MALDI Imaging 
Contact  Rick Drake or Ellen Jones  for a consulation and pricing
 
*
MW Determination ESI    *
MWD by MALDI-TOF MS 1-3 Samples 

1-3 Samples

 
*
MWD by MALDI-TOF MS 4-6 Samples 

4-6 Samples

 
*
Phospho SILAC 

Contact Lauren Ball for consultation and pricing

 
*
Phosphorylation enrichment and analysis    *
Phosphorylation or PTM analysis    *
SILAC 

Contact Lauren Ball for Consulation and Pricing

 
*
Sypro Ruby Gel Stain 10-15cm    *
TMT 

Contact Lauren Ball for consultation and pricing

 
*